expression of spermatogonial and pluripotency markers in spermatogonial stem cells after treatment with different culture factors

Expression of Spermatogonial and Pluripotency Markers in Spermatogonial Stem Cells after Treatment with Different Culture Factors

Background: As condition and component of culture determine fate map of spermatogonial stem cells (SSCs), the aim of this study was to evaluate of growth factors GDNF, LIF and RA on proliferation and differentiation of SSC. Materials and Methods: SSCs were cultured in two groups: The first group GDNF and LIF and the second group RA. The number of clumps and colony formation was monitored dur...

In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells

Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direct induction of ESC-like cells from well-characterized SSCs. Thus, the aim of t...

Spermatogonial Stem Cells: Biology, Isolation, Culture, Characterization, and Practical Perspectives

Spermatogonial stem cells (SSCs) also known as germ stem cells (GSCs) are the basis of spermatogenesis process in the testis. Furthermore, they are also valuable cells with different applications in developmental biology, transgenesis technology, and clinic. Understanding the new findings related to the cell and molecular biology of SSCs and the methods for isolation and maintenance of these ce...

Improvement of Expression of α6 and β1 Integrins by the Co-culture of Adult Mouse Spermatogonial Stem Cells with SIM Mouse Embryonic Fibroblast Cells (STO) and Growth Factors

Effects of nano-selenium on mRNA expression of markers for spermatogonial stem cells in the testis of broiler breeder males

Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects of different levels of Nano-Selenium (Nano-Se) on expression of molecular markers of spermatogon...

Spermatogonial culture medium: an effective and efficient nutrient mixture for culturing rat spermatogonial stem cells.

An economical and simplified procedure to derive and propagate fully functional lines of undifferentiated rat spermatogonia in vitro is presented. The procedure is based on the formulation of a new spermatogonial culture medium termed SG medium. The SG medium is composed of a 1:1 mixture of Dulbecco modified Eagle medium:Ham F12 nutrient, 20 ng/ml of GDNF, 25 ng/ml of FGF2, 100 microM 2-mercapt...